Construction of a Simple Suction Electrode for Extracellular Recording and Stimulation
نویسندگان
چکیده
Principles of signal transmission in nervous systems are commonly demonstrated in the undergraduate neuroscience laboratory through extracellular recording of nerve and muscle action potentials. Here we describe the construction of a simple suction electrode that we use routinely in our laboratory classes for nerve recording and stimulation. The electrode parts are relatively inexpensive, easily available from established scientific and electronic distributors and local hardware stores, and the electrode is resilient to student handling. Our undergraduate students use this electrode design for high resolution, extracellular recordings of action potentials from crayfish motor and sensory nerves and insect muscle, and for stimulation of crustacean and insect motor nerves.
منابع مشابه
Tools for physiology labs: an inexpensive high-performance amplifier and electrode for extracellular recording.
The cost of electronic equipment can be a critical barrier to including neurophysiology exercises in biology teaching programs. We describe the construction of a simple and inexpensive AC preamplifier with performance comparable to that of commercial products. The amplifier consists of two integrated circuits in five stages: differential input, fixed gain, variable gain (100 or 1000), low-pass ...
متن کاملA gold-plated suction electrode for extracellular recording and dye infusion.
To record extracellular nerve activity, wires or polyethylene suction electrodes are commonly used, which are modified according to particular experimental requirements. For electrophysiological studies in a dissected animal, a length of the nerve under investigation may be lifted on to silver wires (Pearon, Stein & Malhotra, 1972), or a fine wire hook (Wilkens & Wolf, 1974), often under minera...
متن کاملSimple techniques suitable for student use to record action potentials from the frog heart.
Demonstrating action potentials during class experiments is very educational for science students. It is not easy, however, to obtain a stable intracellular recording of action potentials from the conventionally used skeletal muscle cells, because the tip of a glass microelectrode often comes out or breaks due to muscle contraction. Here, I present a much simpler recording method using a flexib...
متن کاملRelationship of the P Angle with Stereotactic Arc in Intraoperative Outcome of Patients with Parkinson’s Disease Undergoing Deep Brain Stimulation
Background & Aim: DBS (deep brain stimulation) is a new and successful technique in treatment of symptoms of Parkinsonism especially after drug resistance. Research in this field is mostly designed for evolution of this technique. The present study aimed at evaluating the relationship between the angle formed in midsagittal and STN (sub-thalamic nucleus) axis line and recording l...
متن کاملMulti-electrode arrays technology for the non-invasive recording of neural signals: a review article
The recording of electrophysiological activities of brain neurons in the last half-century has been considered as one of the effective tools for the development of neuroscience. One of the techniques for recording the activity of nerve cells is the multi-electrode arrays (MEAs). Microelectrode arrays (MEAs) are usually employed to record electrical signals from electrogenic cells like neurons o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 6 شماره
صفحات -
تاریخ انتشار 2007